Home  >  Products  >  UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)
UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)

UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)

Cat no: U0880-10C

Supplier: United States Biological
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UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U0880-10C
Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine
Hosts: Goat
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4):483-95.
Additional info: Recognizes UBE2F. Species sequence homology: Bovine, canine, human, mouse, porcine and rat.

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