UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)

Cat no: U0880-10D

Supplier: United States Biological

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UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.05-0.2ug/ml, observed in rat pancreas lysates on ~22kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	U0880-10D
Reactivities:	Human, Mouse, Rat, Bovine, Canine, Porcine
Hosts:	Goat
Applications:	ELISA, Western Blot
Size:	100ug
Form:	Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type:	Pab
Purity:	Purified by immunoaffinity chromatography.
Additional info:	Recognizes rat UBE2F. Species sequence homology: Bovine, canine, human, mouse and porcine.

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UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)

Cat no: U0880-10D

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