UBE2M (Ubiquitin-conjugating Enzyme E2 M, Ubiquitin-protein Ligase M, Ubiquitin Carrier Protein M, Nedd8-conjugating Enzyme Ubc12, NEDD8 Protein Ligase, NEDD8 Carrier Protein, UBC12)

Similar to ubiquitin, Nedd8 signals to target proteins through an enzymatic cascade composed of Nedd8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for Nedd8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 belong to the cullin family of E3 ligases. Neddylation of cullin activates the related ubiquitin E3 complex by promoting its interaction with ubiquitin-E2 ligase and catalyzing downstream target protein ubiquitination (6-7). Neddylation of the cullins on SCFROC1, SCFbTrCP, and SCFSKP2 has been shown to be required for controlling important signaling targets such as IkB, NF-kB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-91E

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Huang, D.T. et al. (2007) Nature 445, 394-8. 2.Huang, D.T. et al. (2005) Mol Cell 17, 341-50. 3.Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14. 4.Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42. 5.Wada, H. et al. (2000) J Biol Chem 275, 17008-15. 6.Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8. 7.Kawakami, T. et al. (2001) EMBO J 20, 4003-12. 8.Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84. 9.Wu, K. et al. (2002) J Biol Chem 277, 516-27. 10.Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9. 11.Herrmann, J. et al. (2007) Circ Res 100, 1276-91.

Additional Info

Recognizes endogeneous levels of total human Ubc12. Species Crossreactivity: Monkey, mouse and rat.