Home  >  Products  >  UBE2N (Ubiquitin-conjugating Enzyme E2N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N, BLU)
UBE2N (Ubiquitin-conjugating Enzyme E2N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N, BLU)

UBE2N (Ubiquitin-conjugating Enzyme E2N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N, BLU)

Cat no: U1000-92J

Supplier: United States Biological
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UBE2N (ubiquitin-conjugating enzyme E2N) is a novel human ubiquitin-conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin-activating enzyme E1-dependent manner. The aa sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. UBE2N protein may be involved in protein degradation mainly in the muscles and testis. Applications: Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 ELISA: 1:5000-1:25,000 Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U1000-92J
Reactivities: Human
Hosts: Goat
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human UBE2N. Species sequence homology: Bovine, chimpanzee, chicken, canine, frog, macaque, mouse, rat and zebrafish.

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