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UBE2N (Ubiquitin-conjugating Enzyme E2N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N, BLU)

Cat no: U1000-92J

UBE2N (Ubiquitin-conjugating Enzyme E2N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N, BLU)

UBE2N (ubiquitin-conjugating enzyme E2N) is a novel human ubiquitin-conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin-activating enzyme E1-dependent manner. The aa sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. UBE2N protein may be involved in protein degradation mainly in the muscles and testis.\n\nApplications: \nSuitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:2000\nELISA: 1:5000-1:25,000\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-92J

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human UBE2N. Species sequence homology: Bovine, chimpanzee, chicken, canine, frog, macaque, mouse, rat and zebrafish.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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