UBE2V1, Active, Recombinant, Human, FLAG-Tag (Ubiquitin-conjugating Enzyme E2 Variant 1, UEV-1, CROC-1, TRAF6-Regulated IKK Activator 1 Beta Uev1A, CROC1, UBE2V, UEV1, P/OKcl.19)

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Multiple alternatively spliced transcripts encoding different isoforms have been described for this gene. A pseudogene has been identified which is also located on chromosome 20. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.\n\nSource:\nRecombinant corresponding to aa2-170 from human UEV1A, fused to FLAG-tag at N-terminal, expressed in E. coli.\n\nMolecular Weight:\n~20kD\n\nApplications: \nSuitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137389

Size

100ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml Flag peptide, 10% glycerol, 3mM DTT.

Purity

Purified (~80%)

References

1. Thomson TM, et al. (1998). FEBS Lett. 423 (1): 49-52. 2. Hau DD, et al. (2006). Biochemistry 45 (32): 9866-77. 3. Ma L, et al. (1998). Oncogene 17 (10): 1321-6.