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UBE2V2, Recombinant, Human, His-tag (Ubiquitin-conjugating Enzyme E2 Variant 2, DDVit-1, EDAF-1, EDPF1, MMS2, Uev2)

Cat no: 145853

UBE2V2, Recombinant, Human, His-tag (Ubiquitin-conjugating Enzyme E2 Variant 2, DDVit-1, EDAF-1, EDPF1, MMS2, Uev2)

Ubiquitin-conjugating Enzyme E2 Variant 2 (UBE2V2), also known as MMS2 and UEV2, is a 145 amino acid (aa) Ubiquitin-conjugating (E2) enzyme variant with a predicted molecular weight of ~16.5kD. The human protein shares 98% and 99% aa sequence identity with the mouse and rat orthologs, respectively. The E2 catalytic core domain of UBE2V2 lacks an active site cysteine residue, rendering it catalytically inactive. However, UBE2V2 can form a catalytically active complex with UBE2N/Ubc13 and this complex specifically catalyzes the formation of Lys63-linked poly-Ubiquitin chains. This complex forms foci in the nucleus following DNA damage and functions in multiple DNA repair pathways. The UBE2N/Ubc13-UBE2V2 complex interacts with the SHPRH and HLTF Ubiquitin ligases (E3s) to promote the poly-ubiquitination of PCNA, a key step in the postreplication repair pathway. Additionally, it functions with the RNF8 E3 to poly-ubiquitinate Histone H2A and Histone H2AX near DNA double-stranded breaks, which is required for the recruitment of DNA repair effector proteins.\n\nSource:\nRecombinant protein corresponding to human UBE2V2, fused to 6-his tag at N-terminal.\n\nMolecular Weight: \n~17.2kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145853

Size

50ug

Form

Supplied as a liquid in 50mM HEPES, pH 7.0, 150mM sodium chloride, 1mM DTT.

Purity

~95% (SDS-PAGE)

References

1. David Y., et al. (2010) J Biol Chem. 285:8595-8604. 2. Bothos J., et al. (2003) Oncogene 22:7101-7107.

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