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UBE3A, Recombinant, Human, His-FLAG-Tag (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, EPVE6AP, HPVE6A, Human Papillomavirus E6-associated Protein, Oncogenic Protein-associated Protein E6-AP, Renal Carcinoma Antigen NY-REN-54)

Cat no: 137390

UBE3A, Recombinant, Human, His-FLAG-Tag (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, EPVE6AP, HPVE6A, Human Papillomavirus E6-associated Protein, Oncogenic Protein-associated Protein E6-AP, Renal Carcinoma Antigen NY-REN-54)

Human UBE3A (var1) aa2-end, with N-terminal His-FLAG tag.\n\nSource:\nRecombinant protein corresponding to human UBE3A, fused to flag-tag, expressed in a Baculovirus infected Sf9 cell expression.\n\nMolecular Weight: \n~99.8kD\n\nApplications: \nSuitable for use in conjunction with E1and E2 for the study of enzyme kinetics, screening\ninhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

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SPECIFICATIONS

Catalog Number

137390

Size

20ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 20% Glycerol, 3mM DTT.

Purity

~81% (SDS-PAGE)

References

1. Dhe-Paganon S., et al Biomol NMR. 51(1-2):185-90. (2011). 2. Jung EM, etal. Int J Mol Med. 28(5):697- 704. (2011).

Alternative Names

EC=6.3.2.-

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