UBF, NT (Nucleolar Transcription Factor 1, Autoantigen NOR-90, Upstream-binding Factor 1, UBF-1, UBTF, UBF1)

The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. UBF binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner. Experiments reveal that a cooperative interaction between UBF and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements which indicate that formation of the UBF-SL1 complex is vital for transcriptional activation by UBF. UBF contains six HMG box domains. The contribution of each HMG box motif to its function is different. Its believed that HMG motifs are the DNA-binding domains of UBF. Altering one of these boxes, as in the second form of UBF, may alter the functional characteristics of the transcription factor. Thus, the existence of different forms of UBF may have important ramifications for transcription by RNA polymerase I.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:30\nWestern Blot: 1:1000-10,000\nImmunohistochemistry: 1:100-1:250\nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa and A431 cell lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029636

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Jantzen, H.M., et al. (1990). Nature. 344(6269):830-836. 2. Bell, S.P., et al. (1988). Science. 241(4870):1192-1197. 3. Yang, W., et al. (2003). Biochemistry. 42(7):1930-1938. 4. O'Mahony, D.J., et al. (1991). Proc Natl Acad Sci. 88(8): 3180-3184.

Additional Info

Recognizes human UBF. Species Crossreactivity: Mouse.