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Ubiquitin Activating Enzyme, E1a (UBE 1a)

Ubiquitin Activating Enzyme, E1a (UBE 1a)

Cat no: U1000-09H

Supplier: United States Biological
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Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the E-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117kD while UBE1b is a nuclear and cytoplasmic protein of 110kD (5). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western blotting 1:1000 Immunoprecipitation 1:50 Immunohistochemistry (Paraffin) 1:175 IHC Protocol Citrate/TBST Immunofluorescence (IF-IC) 1:50 Flow Cytometry 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1000-09H
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.
P type: Pab
Purity: Purified by Protein A and immunoaffinity chromatography.
References: 1. Ciechanover, A. (1998) EMBO J. 17:7151-7160. 2. Hochstrasser, M. (2000) Nat. Cell Biol. 2:E153-E157. 3. Hochstrasser, M. (2000) Science 289:563-564. 4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490:179-189. 5. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608-15614.
Additional info: Detects endogenous levels of total human UBE1a protein. Does not crossreact with UBE1b. Species Crossreactivity: Mouse and rat.

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