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Ubiquitin Aldehyde, Recombinant, Human (rh-Ubiquitin Aldehyde)

Cat no: U1000-06V

Ubiquitin Aldehyde, Recombinant, Human (rh-Ubiquitin Aldehyde)

The C-terminal glycine carboxyl of Ubiquitin is synthetically modified to an aldehyde. Ubiquitin Aldehyde (Ub-H) is useful in the stabilization of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ub-H can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5uM. Co-crystallization of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme: substrate interactions.\n\nSource:\nRecombinant corresponding to human Ubiquitin Aldehyde expressed in E. coli.\n\nMolecular Weight:\n~8.5kD\n\nBiological Activity: Ki = 2.5nM vs UCH isopeptidase-T\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-06V

Size

50ug

Form

Supplied as a liquid in an aqueous solution, 0.15M hydrochloride.

Purity

~95% (HPLC, SDS-PAGE).

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