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Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human (E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UbcH5, UBCH5, UbcH5A, UBCH5A, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-

Cat no: U1000-86N

Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human (E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UbcH5, UBCH5, UbcH5A, UBCH5A, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-

Source:\nRecombinant corresponding to aa2-end of human UbcH5a with N-terminal HIS tag expressed in E. coli (NM_003338).\n\nSpecific Activity:\n50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, UBE1 (0.5ug), UbcH5a or other E2, Biotin-Ubiquitin (0.5ug) and Smurf2 (2) in 50mM Tris pH 7.4 for 30 min at 37 degrees C, then add to streptavidin coated plates for 30 min at 37 degrees C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (lambda-ex=330nm, lambda-em=620nm).\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Aliquots are stable for at least 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-86N

Size

100ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.

Purity

Purified (same/more than)80%

References

1. Markson G. et al., Genome Res. 19, 1905-1911 (2009). 2. Nuber U. and Scheffner M., J. Biol. Chem. 274(11), 7576-7582 (1999).

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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