Home  >  Products  >  Ubiquitin-conjugating Enzyme E2 D2, Recombinant, Human, His-tag (UBE2D2, UBC4, UBC5B, UBCH4, UBCH5B, Ubiquitin Carrier Protein D2, Ubiquitin-conjugating Enzyme E2(17)KB 2, Ubiquitin-conjugating Enzyme E2-17kD 2, Ubiquitin-protein Ligase D2)

Ubiquitin-conjugating Enzyme E2 D2, Recombinant, Human, His-tag (UBE2D2, UBC4, UBC5B, UBCH4, UBCH5B, Ubiquitin Carrier Protein D2, Ubiquitin-conjugating Enzyme E2(17)KB 2, Ubiquitin-conjugating Enzyme E2-17kD 2, Ubiquitin-protein Ligase D2)

Cat no: U1000-86Q


Supplier: United States Biological
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UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100nM to 1uM depending upon the conditions. Source: Recombinant corresponding to human UbcH5b with His-tag, expressed in E. coli. Molecular Weight: ~17kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U1000-86Q
Size: 100ug
Form: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.
Purity: ~95% (SDS-PAGE)
Alternative names: EC=6.3.2.19

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