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Ubiquitin-conjugating Enzyme E2 H, Recombinant, Human, His-tag (UBE2H, UbcH2, Ubiquitin Carrier Protein H, Ubiquitin-conjugating Enzyme E2-20K, Ubiquitin-protein Ligase H)

Cat no: U1000-87A1

Ubiquitin-conjugating Enzyme E2 H, Recombinant, Human, His-tag (UBE2H, UbcH2, Ubiquitin Carrier Protein H, Ubiquitin-conjugating Enzyme E2-20K, Ubiquitin-protein Ligase H)

UbcH2 is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UbcH2 consists of two functionally independent domains, an N-terminal core domain with ubiquitin conjugating activity, and a C-terminal domain that interacts with substrate proteins. This protein is useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM depending upon conditions.\n\nSource:\nRecombinant corresponding to human UbcH2 with His-tag, expressed in E. coli.\n\nMolecular Weight:\n~22.2kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-87A1

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.

Purity

~95% (SDS-PAGE)

Alternative Names

EC=6.3.2.19

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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