Ubiquitin Conjugating Enzyme E2 Variant 1 Isoform a (UBE2V1a, UBE2V1, UBE2V, UEV1A, UEV1, UEV-1, CIR1, CROC1, CROC-1, P/OKcl.19, TRAF6-regulated IKK Activator 1 beta Uev1A)

The E2 enzyme, Ubc13, and the E2 enzyme variants, Uevs, form stable, high affinity complexes for the assembly of Lys63-linked ubiquitin chains. This complex has been shown to activate NF-kB signaling pathway by linking TRAF6 to IKKs. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results suggest a new regulatory function for ubiquitin, in which IKK is activated through the assembly of Lys63-linked polyubiquitin chains. The UEV proteins are also involved in the control of differentiation.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat, HeLa or RAW (mouse) cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-75A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Deng,L., Wang,C., Spencer,E., Yang,L., Braun,A., You,J., Slaughter,C., Pickart,C. and Chen,Z.J. Activation of the IkB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 103 (2), 351-361 (2000). 2. Sancho,E., Vila,M.R., Sanchez-Pulido,L., Lozano,J.J., Paciucci,R., Nadal,M., Fox,M., Harvey,C., Bercovich,B., Loukili,N., Ciechanover,A., Lin,S.L., Sanz,F., Estivill,X., Valencia,A. and Thomson,T.M. Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells. Mol. Cell. Biol. 18 (1), 576-589 (1998). 3. Rothofsky,M.L. and Lin,S.L. CROC-1 encodes a protein which mediates transcriptional activation of the human FOS promoter. Gene 195 (2), 141-149 (1997).

Additional Info

Recognizes human UBE2V1a. Species sequence homology: mouse and rat.