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Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)

Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)

Cat no: U1000-90A

Supplier: United States Biological
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Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1000-90A
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied in liquid in PBS, pH 7.4, 0.1% sodium azide.
P type: Mab
Isotype: IgG2b,k
Purity: Purified by immunoaffinity chromatography.
References: 1.Ardley HC., et al. (2000) Cytogenet Cell Genet. 89(1-2):137-140 2.Movnihan TP., et al. (1999) J Biol Chem. 274(43):30963-30968 3.Kumar S., et al. (1997) J Biol Chem. 272(21):13548-13554
Additional info: Recognizes human UBE2L6 (1-152aa)

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