Home  >  Products  >  Ubiquitin Conjugating Enzyme E2N (Ubiquitin-conjugating Enzyme E2 N, UBE2N, Bendless-like Ubiquitin-conjugating Enzyme, BLU, MGC8489, MGC131857, UBC13, UbcH-ben, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N)
Ubiquitin Conjugating Enzyme E2N (Ubiquitin-conjugating Enzyme E2 N, UBE2N, Bendless-like Ubiquitin-conjugating Enzyme, BLU, MGC8489, MGC131857, UBC13, UbcH-ben, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N)

Ubiquitin Conjugating Enzyme E2N (Ubiquitin-conjugating Enzyme E2 N, UBE2N, Bendless-like Ubiquitin-conjugating Enzyme, BLU, MGC8489, MGC131857, UBC13, UbcH-ben, Ubiquitin Carrier Protein N, Ubiquitin-protein Ligase N)

Cat no: U1000-92H

Supplier: United States Biological
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Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway. It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human small intestine or HepG2 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U1000-92H
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: Ubiquitin-conjugating Enzyme E2N (UBC13 Homolog, Yeast), EC=6.3.2.19
References: 1. Brusky J, Zhu Y, and Xiao W. UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr. Genet. 2000; 37:168-74. 2. Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511. 3. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 4. Deng L, Wang C, Spencer E, et al. Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61. 5. Wang C, Deng L, Hong M, et al. TAK1 is a ubiquitin-dependent kinase of MKK and IKK. Nature 2001; 412:346-51. 6. Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-
Additional info: Recognizes human UBC13. Species Crossreactivity: mouse and rat.

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