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Ubiquitin, Recombinant, Human

Cat no: U1000-06F

Ubiquitin, Recombinant, Human

Ubiquitin (Ub) is a small protein that is composed of 76aa. Ub is found only in eukaryotic organisms and is highly conserved. Ub can exist either in free form or as part of a complex with other proteins. In the latter case, Ub is attached (conjugated) to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains of lysine on the proteins; Ubiquitination. Functions to regulate protein turnover in a cell by closely regulating the degradation of specific proteins. Ubiquitin has been immunohistochemically localized to a number of pathological inclusions, including ; Lewy bodies of Parkinson's disease,neurofibrillary of Alzheimer's disease, Pick bodies of Pick's disease.\n\nSource: \nRecombinant corresponding to aa1-76, with N-GST tag, from human Ubiquitin, expressed in E.coli.\n\nMolecular Weight:\n~35kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-06F

Size

500ug

Form

Supplied as a lyophilized powder from 20mM HEPES, pH 7.4. Reconstitute with sterile dH2O PBS.

Purity

~95% (SDS-PAGE)

References

1. Ross CA, et al. (2004) Trends Cell Biol. 14 (12) :703-11. 2. Hattori N, et al.(2004) Lancet. 364(9435):722-4.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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