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Ubiquitin (RPS27A, UBA52, UBB, UBC)

Cat no: U1000-07E

Ubiquitin (RPS27A, UBA52, UBB, UBC)

Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56kD which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry (formalin-fixed, paraffin-embedded): 1:150 -1:300\nELISA: Indirect\nOptimal dilutions to be determined by the researcher. \n\nRecommended Positive Control:\nEndometrial Tissue Homogenates\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-07E

Size

250ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat, NHP

Form

Supplied as alyophilized powder in PBS, pH7.2, 100mM sodium chloride, 15mM sodium azide. Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes ubiquitin. Does not react with KLH alone. Species Crossreactivity: human, baboon, and rat. Species sequence homology: bovine.

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