Ubiquitin

Ubiquitin is a 76-amino acid polypeptide expressed in all eukaryotic cells and it is highly conserved from yeast to humans (1). The modification of proteins by chains of ubiquitin has long been known to mediate targeting of cytosolic and nuclear proteins for degradation by proteasomes. Ubiquitination is an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). E1 activates ubiquitin in an ATP-dependent manner with the formation of a thiol-ester linkage between the carboxy terminus of ubiquitin and E1. Sequential, transient thiol-ester bonds are then generated between the carboxyl terminus of ubiquitin and specific cysteines of E2 and E3 enzymes (2). These bonds culminate in the formation of an isopeptide linkage between the activated carboxy terminus of ubiquitin and the epsilon-amino group of a lysine on a target protein or within another ubiquitin chain (usually K48 of ubiquitin) resulting in the generation of chains of ubiquitin (polyubiquitin or multiubiquitin) which may be from 4

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

U1000-01E

Size

50ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, GP, NHP, Prc, Rabbit, Sheep, Xen/Amph, Yeast/Fun

Form

Suppled as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 2. Bonifacino, J. S. and Weissman, A. M. (1998) Annu. Rev. Cell Dev. Biol. 14:19-57. 3. Yang, M., Omura, S., Bonifacino, J.S., and Weissman, A.M. (1998) J. Exp. Med. 187:1835-46. 4. Fisher, E.A., et al., (1997) J. Biol. Chem. 272:20427-20434. 5. de Virgilio, M., Weninger, H.and Ivessa, N.E. (1998) J. Biol. Chem. 273:9734-9743. 6. Mallampalli, R.K., et al. (2000) J. Biol. Chem. 275:9699-9708. 7. Le Petit-Thevenin, J., et al., (2001) Biochemica et Biophysica Acta. 1530:184-198. 8. Kang, B.-S., French, O.G., Sando, J.J., Hahn, C.S. (2000). Oncogene. 19:4263-4272. 9. Nakanishi, Y. et al (2000). Am. J. Respir. Cell Mol. Biol. 22:747-754.

Additional Info

Recognizes bovine Ubiquitin at ~10kD, corresponding to the apparent molecular mass of ubiquitin on SDS-PAGE immunoblots.\nSpecies Crossreactivity: human, monkey, mouse, rat, hamster, rabbit, guinea pig, porcine, canine, sheep, chicken, Xenopus, yeast, Drosophila, fish (rainbow trout).