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Ubiquitin

Cat no: U1000-01K


Supplier: United States Biological
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Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:25-1:500 Western Blot: 1:2500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1000-01K
Reactivities: Rabbit
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. von Mikecz A. (2006) .J. Cell. Sci. May 119(Pt10):1977-1984. 2. Xu G, et al. (2005) J. Biol. Chem. Dec 280(52):42841-42847. 3. Jain M, Das RH. (2004) Mol. Biol. Rep. Dec 31(4):261-266.
Additional info: Detects ubiquitinated inclusion bodies such as neurofibrillary tangles of Alzheimer's disease, Lewy bodies of Parkinson's disease, Pick bodies of Pick's disease. Works well on formalin fixed material.

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