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Ubiquitin

Cat no: U1000-02

Ubiquitin

Ubiquitin is a 76 amino acid, highly conserved protein of about 8.5kD which is highly conserved evolutionarily. All plant and animal species are observed to express ubiquitin. Ubiquitin is produced in response to stress. The formation of ubiquitin-protein conjugates is a signal for the selective degradation of proteins. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Ubiquitin has been immunohistochemically localized to a number of pathological inclusions, including: Lewy bodies of Parkinson

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SPECIFICATIONS

Catalog Number

U1000-02

Size

100ul

Applications

IHC, WB

Hosts

Sheep

Reactivities

Hum, Bov, Rabbit, Sheep

Form

Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul sterile ddH2O or sterile 40-50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Haas, et al., J. Biol. Chem. 260: 12,464-12,474 (1985).

Additional Info

Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western Blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections. Species Crossreactivity: Human, bovine, rabbit and sheep.

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