Ubiquitin

Cat no: U1000-07J

Supplier: United States Biological

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Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more than one ubiquitin monomers. Ubiqutination controls the protein degradation, stability and intracellular localization of many proteins. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.12-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	U1000-07J
Hosts:	Mouse
Applications:	Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	25ug
Form:	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type:	Mab
Isotype:	IgG1,k
Purity:	Purified by immunoaffinity chromatography.
References:	1. Kahana, et al. 1999. Mol. Cell Biol 19:6608. 2. Ciechanover A, et al. 1998. EMBO J 17:7151. 3. Xu P, et al. 2008. Analytical Chemistry 80:3438.
Additional info:	Recognizes ubiquitin in all species.

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Ubiquitin

Cat no: U1000-07J

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