Ubiquitin (Ubiquitin B, UBB, hCG_1998947) (Biotin)

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-06S-Biotin

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Chan, Y.L., et al., Biochem. Biophys. Res. Commun. 215(2):682-690 (1995). 2.Cook, W.J., et al., J. Mol. Biol. 236(2):601-609 (1994). 3.Hubbard, M.J., et al., Biochim. Biophys. Acta 1200(2):191-196 (1994). 4.Wajih, N., et al., Protein Seq. Data Anal. 5(1):31-32 (1992). 5.Cook, W.J., et al., J. Biol. Chem. 267(23):16467-16471 (1992).

Additional Info

Recognizes human Ubiquitin.