UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5) (Azide free) (HRP)

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230aa human UCHL3 protein is 54% identical to that of UCHL1.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1005-01L-HRP

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.M-P. et al. Proteomics. July; 9(13): 3609-3622(2009). 2.Hu, R.M., et al., Proc. Natl. Acad. Sci. USA 97(17):9543-9548 (2000). 3.Lai, C.H., et al., Genome Res. 10(5):703-713 (2000).

Additional Info

Recognizes human UCHL5. Species Crossreactivity: mouse.