Home  >  Products  >  UEV2 (Ubiquitin Conjugating Enzyme E2 Variant 2, UBE2V2, 1 alpha,25-dihydroxyvitamin D3-inducible, DDVIT1, EDAF1, EDPF1, Enterocyte Differentiation Promoting Factor, Methyl Methanesulfonate Sensitive 2, MMS2)
UEV2 (Ubiquitin Conjugating Enzyme E2 Variant 2, UBE2V2, 1 alpha,25-dihydroxyvitamin D3-inducible, DDVIT1, EDAF1, EDPF1, Enterocyte Differentiation Promoting Factor, Methyl Methanesulfonate Sensitive 2, MMS2)

UEV2 (Ubiquitin Conjugating Enzyme E2 Variant 2, UBE2V2, 1 alpha,25-dihydroxyvitamin D3-inducible, DDVIT1, EDAF1, EDPF1, Enterocyte Differentiation Promoting Factor, Methyl Methanesulfonate Sensitive 2, MMS2)

Cat no: U1075-10A

Supplier: United States Biological
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The differentiation and activation of monocytes (MO) and monocytic cells is modulated by Vitamin D3. Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5kD protein. The product of this gene has structural homology with yeast MMS2P and human CROC-1, which defines a structurally and functionally conserved UBC-like protein family. This protein was called UEV2. The yeast UEV protein is required together with UBC13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins also play a central role in the assembly of K63-linked polyubiquitin chains UEV/UBC complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the UEV2 heterodimer is necessary for DNA repair. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1075-10A
Reactivities: Human
Hosts: Goat
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Fritsche J et al. Molecular cloning of a 1alpha,25-dihydroxyvitamin D3-inducible transcript (DDVit 1) in human blood monocytes. Biochem Biophys Res Commun 235:407-12 (1997).
Additional info: Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Rat and Mouse due to sequence homology.

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