UGRP2, Recombinant, Mouse (Secretoglobin Family 3A Member 1, Cytokine HIN-1, High in Normal 1, Pneumo Secretory Protein 2, PnSP-2, Uteroglobin-related Protein 2, HIN1, PNSP2, SCGB3A1, UNQ629/PRO1245)

High in Normal-1 also known as Uteroglobulin related protein 2 (UGRP2), is an 8kD secreted protein of the secretoglobin superfamily (SCGB3A1). HIN-1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate. It is up-regulated in Clara cells by IL-4, IL-13, Oncostatin M, EGF, and TGF-a and down-regulated by IFNa B, and g. It is secreted into bronchial lavage fluid, saliva, and plasma and may form disulfide-linked dimers. HIN-1 binds to the macrophage scavenger receptor MARCO and to the surface of mammary and bronchial epithelial cells. HIN-1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells likely through the Akt signaling pathway. The down-regulation of HIN-1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter. Mature mouse HIN-1 shares 58% and 93% aa sequence identity with human and rat HIN-1, respectively. A variant of mouse HIN-1 has a substitution at the N-terminus in place of its potential signal peptide.\n\nSource:\nRecombinant corresponding to aa22-104 from mouse HIN-1expressed in E.coli. \n\nMolecular Weight:\n~8.4kD\n\nActivity:\nMeasured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. The ED50 for this effect is typically 0.5-2.5ug/ml\n\nEndotoxin: ~1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

U7001-21

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with sterile PBS to 0.25mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707. 2.Porter, D. et al. (2002) Mech. Dev. 114:201. 3. Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med.166:1498. 4. Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796. 5. Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120. 6. Yamada, A. et al. (2005) J. Immunol. 175:5708. 7.Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620. 8. Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221. 9. Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964. 10. Krop, I. et al. (2004) Mol. Cancer Res. 2:489. 11. Bin, L.-H. et al. (2003) J. Immunol. 171:924. 12. Krop, I. et al. (2005) Cancer Res. 65:9659. 13. Krop, I. et al. (2003) Cancer Res. 63:2024. 14. Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.