Uncoupling Protein 1 (UCP1)

The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxoglutarate/malate and the citrate (tricarboxylate) carriers. UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold- and diet induced thermogenesis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide: \nU1620-15: Uncoupling Protein 1, Mouse, Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1620-18

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kozak LP et (1988) JBC 263:12274. 2. Bouillaud F (1986) JBC 261:1487. 3. Ridley RG et a (1986) Nucl. Acid Res. 14:4025. 4. Miroux B et al (1993) EMBO J 12:3739. 5. Cassard AM et al (1990) JCB 43:255. 6. Fleury C et al 91997) Nature Genetics 15:269-272. 7. Feil S and Rafael J (1994) Eur. J. Biochem. 219:681. 8. Boss O et al (1997) FEBS Lett. 408:39-42.

Additional Info

Recognizes the mouse Uncoupling Protein 1. The UCP1 peptide has no significant homology with UCP2-5.