Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

The regulation of body weight depends upon calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic.\nMouse UCP2 is a 309aa (human 309aa chromosome 7; ~95% homology) mitochondrial uncoupling protein (1). It is only 59% homologous with UCP1 found in BAT. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, BAT, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation (1,2).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well. See catalog # U1621-06.\nWestern Blot: 1:1000-1:5000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1621-11

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Fleury, C., et al., Nature Genetics 15: 269-272 (1997). 2. Flier, J.F. and Lowell, B.B., Nature Genetics 15: 223-224 (1997). 3. Boss, O., et al., FEBS Lett. 408: 39-42 (1997).

Additional Info

Recognizes the mouse Uncoupling Protein 2 (UCP2, SLC25A8, UCPH). The UCP2 peptide has no significant homology with UCP1, UCP3, UCP4 or UCP5.