Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein (1). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nWestern Blot: 1-10ug/ml (ECL)\nImmunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue. Reported to detect UCP2 in human neuroblastoma cell shy cells.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide: \nU1621-12: Uncoupling Protein 2, Human, Control Peptide (UCP2, SLC25A8, UCPH)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1621-13

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fleury C et al (1997) Nature Genetics 15: 269-272. 2. Flier JF and Lowell BB (1997) Nature Genetics 15: 223-224. 3. Boss O et al (1997) FEBS Lett. 408:39-42. 4. Fridell Y-W C (2005) Cell Metabol. 1:145-152. 5. Mitchell JB (2003) Free Radical Biol. Med. 34: 93-102 (WB). 6. Mark FC (2006) Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 1: 365-374 (WB). 7. Ho W-L (2005) J. Neurosci. 81: 261-268 (WB, IHC).

Additional Info

Recognizes human UCP2. No significant homology with UCP1 or UCP3-5.