Uncoupling Protein 3 (UCP3)

The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). \n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:10,000-1:100,000 using 50-100ng UCP31 control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nU1622-01 Uncoupling Protein 3, Human Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

U1622-02

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gustafsson H, 2004, J. Neurochemistry 88, 2:462-468, 2. Guillet-Deniau I, 2002, Diabetes 51:1722-1728 3. Marcinek DJ, 2005, The Journal of Physiol. 569:467-473, 4. Jucker BM, 2000, Proc. Natl. Acad. Sci. 97: 6880-6884 5. Murray AJ, 2004, The Lancet, 364, 9447, 1786-1788 6. Gerber LK, 2006, Am J Physiol Cell Physiol, 291: C1198 - C1207 7. Guerini D, 2002, Eur. J. Biochem. 269:1373-1381 8. Barazzoni R, 2001, Am J Physiol. Endocrinol. Metabol. 280, 413E 9. Pedraza N, 2000, Diabetes 49:1224-1230 10. Clapham JC, 2000, Nature 406:415 - 418 11. McFalls EO, 2006, J of Molecular and Cellular Cardiology, 41, 980- 12. Murray AJ, 2005, Diabetes, Dec 2005; 54:3496 - 3502 13. Jimenez M, 2002, Eur. J. Biochem. 269:2878-2884 14. Cheng L, 2004, Nature Med.10, 1245 - 1250 15. Boss, O et al (1997) FEBS Lett. 408:39-42. 16. Vidal-Puig A et al (1997) BBRC 235:79-82.

Additional Info

Recognizes human Uncoupling Protein 3 (UCP3). Species sequence homology: monkey-88%, humming bird-78%, canine, porcine-76%, mouse, bovine, hamster-70%, and rat-64%.