Uncoupling Protein 5, Rat, Control Peptide (UCP5, BMCP1)

Control Peptide for U1625-03 (antiserum) and U1625-01 (affinity purified antibody). Synthetic peptide 16aa sequence within the N-terminus of the first extracellualr region of rat UCP5. The sequence is conserved in all 3 isoforms in rat and mouse UCP5. No significant sequence homology with UCP1-4. Species crossreactivity: Rat. Species Sequence Homology: Mouse: 100%; human UCP5/BMCP1: 94%.\n\nThe regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three mitochondrial energy transfer protein domains (ETPDs). Both N- and C-termini are predicted to be cytoplasmic. UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22aa N-terminal sequence; UCP5S is missing 3aa (23-25aa) and UCP5SI has 31aa insertion between TM II-IV.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng/well.\nAntibody Blocking: 5-10ug Control Peptide per 1ug U1625-01 (affinity purified antibody) or per 1ul U1625-03 (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1625-14

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Purity

Highly purified

References

1. Yu XX et al (2000) FASEB J. 14:1611-1618. 2. Sanchis (2000) FASEB J. 14:1611-1618. 3. Sanchis D et al (1998) JBC 273:34611-34615. 4. Kim-han JS et al (2001) J. Neurochem. 79:658-665.