The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. UBF binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner (1). Experiments reveal that a cooperative interaction between UBF and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements which indicate that formation of the UBF-SL1 complex is vital for transcriptional activation by UBF (2). UBF contains six HMG box domains. The contribution of each HMG box motif to its function is different (3). Its believed that HMG motifs are the DNA-binding domains of UBF. Altering one of these boxes, as in the second form of UBF, may alter the functional characteristics of the transcription factor. Thus, the existence of different forms of UBF may have important ramifications for transcription by RNA polymerase I (4)
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:30
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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