Uracil-DNA Glycosylase 2 (Cyclin-O, Uracil-DNA Glycosylase 2, UDG2, Cyclin-like Uracil-DNA Glycosylase, CCNO, UDG2, UNG2)

UNG2 is a 38kD member of the cyclin family, expressed in the nucleus. Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine. UNG2 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity. In association with activation-induced cytosine deaminase, UNG2 is also essential to the generation of strand breaks that initiate class switch recombination.\n\nUNG2 is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1900-20C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hagen, L. et al. (2006) Genomic uracil and human disease. Exp Cell Res. 312:2666-2672. 2. Krokan, H. et al. (2001) Properties and functions of human uracil-DNA glycosylase from the UNG gene. Prog. Nucleic Acid Res. Mol. Biol. 68:365-386.

Additional Info

Recognizes human UNG2. Species Crossreactivity: rat, mouse.