Uracil-DNA glycosylase (UNG) is a highly conserved DNA repair enzyme which exists as both a mitochondrial (UNG1) and nuclear (UNG2) isoform, generated from alternative promoter usage and gene splicing.
UNG is essential in the base excision repair pathway for the specific recognition and removal of the RNA base uracil from DNA, resulting from cytosine deamination or misincorporation of dUTP. UNG is known to be encoded by Herpes viruses and poxviruses, and has been shown to associate with both Vpr and integrase (IN) in immunodeficiency virus type 1 (HIV-1) particles.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1,000-1:2,000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.