Urate Transporter 1 (URAT1)

Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500 uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~60 kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12-TM domains with cytoplasmic N and C-terminus. URTA1 is prominent is epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 using ECL technique\nELISA: 1:10,000-50,000. Coat ELISA plate with 1ug/ml control peptide.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: U2005 \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2005-01

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Entomoto A et al (2002) Nature 417:447-452. 2. Mori K et al (1997) FEBS Lett. 417:371-374.

Additional Info

Recognizes Urate Transporter 1 (URAT1). Peptide Sequence Homology: human URAT1/RST or OAT-4-like protein: 100%; mouse RST/URAT1: 54%.; no significant homology with other OAT4 or other proteins. \n\n