Urate Transporter 1 (URAT1)

Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~40kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12 transmembrane domains with a cytoplasmic N- and C-terminus. URAT1 is prominent in epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1-10ug/ml using ECL.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nU2005: Urate Transporter 1, Human (URAT1) (Control Peptide)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2005-05

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Entomoto, A., et al., Nature 417: 447-452 (2002). 2. Mori, K., et al., FEBS Lett. 417: 371-374 (1997). 3. Price, K.L., et al., J. Am. Soc. Nephrol. 17: 1791-1795 (2006).

Additional Info

Recognizes human URAT1. Species Sequence Homology: Mouse RST/URAT1 (54%). No significant sequence homology is observed with other OAT4 or other proteins.