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Urea BioAssay(TM) Kit

Cat no: U2010-01

Urea BioAssay(TM) Kit

Urea is a waste product which produced in the liver, dissolved in blood (in a concentration of 2.5-7.5mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. Urea determination is very useful for the medical clinician to assess kidney and other organs function of patients. \n\nUrea BioAssay(TM) Kit provides a rapid, simple, sensitive, and reliable for measurement of Urea level in a variety of samples such as serum, plasma, and urine, etc. In the assay, Urea reacts as substrate with compounds in the presence of enzymes to form a product that reacts with the OxiRed probe to generate color (Lmax 570nm). The optical density of produced color has a direct relationship with Urea concentration in the solution. The kit can detect as low as 0.5nmol/well or 10uM of Urea. The assay is also suitable for high throughput studies.\n\nKit Components: \n1. Urea Assay Buffer: 1x25ml \n2. Oxidation/Reduction Probe in DMSO: 1x25ul\n3. Enzyme Mix (lyophilized): 1x400ul \n4. Developer: 1x1 vial \n5. Converting Enzyme: 1x1 vial \n6. Urea Standard (100mM): 1x100ul \n\nStorage and Stability:\nStore kit at -20 degrees C. Protect from light. Allow Assay Buffer to warm to room temperature \nbefore use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. All the solution in this kit should be kept capped when not being used to prevent absorption of NH3 from the air

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SPECIFICATIONS

Catalog Number

U2010-01

Size

100Tests

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