Urease, Recombinant

The active mutant urease is expressed in E. coli by recombinant DNA procedures and purified by conventional chromatographies. Mutant urease from Klebsiella aerogenes has a vast Km value (200mM). The substitution of His219 at the active center of a subunit to other amino acid residues by protein engineering is known to modify the affinity against urea and to shift Km. Among those mutants, aH219Q has almost similar enzymatic properities except for a vast Km against urea. The Km value is 200mM, being 1000 fold larger than that of wild type (0.2mM).\n\nReaction Equation: Urea + H2O + 2H+ = 2NH4+ +CO2\n\nBiological Activity: As reported \n\nSpecific Activity: ~150u/mg protein (25 degrees C, pH 7.0).\n\nStandard: Recombinant Urease-03\n\nContaminant: (as Recombinant Urease activity = 100%) NADPHox (same/less than) 0.001%\n\nOne mg of powder contains the following: \nProtein 500

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SPECIFICATIONS

Catalog Number

U2015-01A

Size

1000U

Form

Supplied as lyophilized powder.

References

1. Beckers G, et al., (2004) J Bacteriol Nov;186(22):7645-52. 2. Nakano M, Iida T, (2004) Microbiology Oct;150(Pt 10):3483-9. 3. Tarun EI, Rubinov DB, (2004) Prikl Biokhim Mikrobiol Jul-Aug;40(4):398-406. 4. Berdoz J, Corthesy B, (2004) Mol Immunol Aug;41(10):1013-22. \n5. Rodriguez BB, Bolbot JA, (2004) Anal Bioanal Chem Sep;380(2):284-92. 6. Suzuki H, et al., (2004) Aliment Pharmacol Ther Jul;20(Sup1):185-92.