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Urease (Urea Amidohydrolase)

Cat no: U2015

Urease (Urea Amidohydrolase)

Urease occurs in many bacteria, several species of yeast and a number of higher plants. Two of the best sources are Jack beans (Canavlia ensiformis) from which it has been crystallized and thoroughly studied, and Bacillus pasteurii. Urease catalyzes the hydrolysis of urea. The molecular weight of the jack bean enzyme is 480,000 daltons, with an optimum pH of 6.0. It is inhibited by heavy metals.\n\nUnit Definition: \n1 unit oxidizes one micromole of NADH per minute at 25C, pH 7.6. The hydrolysis of urea is measured by coupling ammonia production to a glutamate dehydrogenase reaction.\n\nActivity: (same/more than)45 units per mg dry weight\n\nA280:\nAs reported\n\nAmmonia:\nAs reported\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2015

Size

250mg

Form

Supplied as a lyophilized powder.

References

1. Guilbault, G. G. and Montalvo, J. B., (1970) J. Amer. Chem. Soc., 92:2533.

Alternative Names

EC=3.5.1.5; Jack bean

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