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Ureb1 (Upstream Regulatory Element Binding Protein 1, ARF Binding Protein 1, ARF BP1, ARF-BP1, BJ-HCC-24 Tumor Antigen, E3 Ubiquitin Protein Ligase HUWE1, HECT UBA and WWE Domain-containing Protein 1, HUWE, HECTH9, HSPC272, Ib772, KIAA0312, KIAA1578, LASU

Cat no: U2020-01B

Ureb1 (Upstream Regulatory Element Binding Protein 1, ARF Binding Protein 1, ARF BP1, ARF-BP1, BJ-HCC-24 Tumor Antigen, E3 Ubiquitin Protein Ligase HUWE1, HECT UBA and WWE Domain-containing Protein 1, HUWE, HECTH9, HSPC272, Ib772, KIAA0312, KIAA1578, LASU

Upstream Regulatory Element Binding Protein 1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000\nImmunoprecipitation: 2-5ug/mg lysate\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa whole cell extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U2020-01B

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris-citrate/phosphate pH 7.5, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Noy T, Suad O, Taglicht D, Ciechanover A. HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation. Biochem Biophys Res Commun. 2012 Feb 10;418(2):408-13. Epub 2012 Jan 18. [PMID: 22277673]

Additional Info

Recognizes human Ureb1. The epitope maps to a region between residues 2250-2300 of human Ureb1 (NP_113584.3) (GeneID 10075).

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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