Urinary Creatinine Detection Kit (2 Plate Kit)

Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate for the production of ATP. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. Under normal conditions, its formation occurs at a rate that is relatively constant and so creatinine is a useful tool for normalizing the levels of other molecules found in urine A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear microtiter plate. The color generating reaction is initiated with the Creatinine Reagent added to all standards, controls and samples. After a short incubation the intensity of the generated color is read in a microtiter plate reader at 490nm. This assay has been validated for diluted human urine samples. The end user should evaluate recoveries of creatinine in other species being studied. to a NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear microtiter plate. The color generating reaction is initiated with the DetectX(R) Creatinine Reagent added to all standards, controls and samples. After a short incubation the intensity of the generated color is read in a microtiter plate reader at 490nm. Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate for the production of ATP. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. Under normal conditions, its formation occurs at a rate that is relatively constant and so creatinine is a useful tool for normalizing the levels of other molecules found in urine. Sample Types This assay has been validated for diluted human urine samples. The end user should evaluate recoveries of creatinine in other species being studied.