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Urokinase, Human Urine

Cat no: U2605-01

Urokinase, Human Urine

Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. UK is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54kD.\n\nSpecific Activity:\nhUK conforms an activity greater than 68,000IU/mg.\n\nActivity:\n1nM UK will cause a change in absorbance of 0.001 at 405nm in 1 minute at RT in 100ul 0.05M Tris-HCl, 0.1M sodium chloride, pH 7.4, using S2444 as the substrate.\n\nContaminants:\nFree of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2605-01

Size

100ug

Form

Supplied as a lyophilized powder from PBS. Reconstitute with sterile ddH2O to >0.1mg/ml.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. De Sio L, et al., (2004) Pediatr Infect Dis J 23:963-965. 2. Das, A, et al., (2004) Arch Ophthalmol 122:1844-1849. 3. Jorgensen, T.J., et al., (2004) Biochemistry 43:15044-15057. 4. Naka, T., et al., (2004) Am J Clin Pathol 122:926-930. 5. Kwak, S.M., et al., (2004) Yonsei Med J 45:822-828. 6. de Paulis, A., et al., (2004) J Immunol 173:5739-5748.

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