The AutoFlow model NU-4750 US CO2 water-jacketed Incubator has been designed to provide a reliable controlled in-vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and preservation of embryos, gametes and animal tissue cell cultures at or near body temperature, containing all five parameters that contribute to optimum growth conditions; Humidity, Precise temperature control, Precise CO2 control, Sterility, and Reliability.