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USF2 (Upstream Stimulatory Factor 2, Major Late Transcription Factor 2, Upstream Transcription Factor 2)

USF2 (Upstream Stimulatory Factor 2, Major Late Transcription Factor 2, Upstream Transcription Factor 2)

Cat no: U1740-02A

Supplier: United States Biological
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The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43kD (USF-1) and 44kD (USF-2) polypeptides, which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loophelix domains. USF also contains a leucine repeat that is required for efficient DNA binding. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min Optimal dilutions to be determined by the researcher. Positive Control: NIH3T3 cells, breast carcinoma Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U1740-02A
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes mouse USF-2. Species Crossreactivity: human and rat.

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