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USP13 (Ubiquitin Carboxyl-terminal Hydrolase 13, Ubiquitin Thiolesterase 13, Ubiquitin-specific Processing Protease 13, Deubiquitinating Enzyme 13, Isopeptidase T-3, ISOT-3, ISOT3)

Cat no: U4100-22B

USP13 (Ubiquitin Carboxyl-terminal Hydrolase 13, Ubiquitin Thiolesterase 13, Ubiquitin-specific Processing Protease 13, Deubiquitinating Enzyme 13, Isopeptidase T-3, ISOT-3, ISOT3)

PHD2 (Egl nine homolog 1) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in PHD2 are associated with erythrocytosis familial type 3 (ECYT3).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:100-1:500\nWestern Blot: 1:1,000-1:10,000\nImmunocytochemistry: 1:100-1:250\nImmunoprecipitation: 1:10-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

U4100-22B

Size

100ul

Applications

FC, ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

References

1. The UniProt Consortium. The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010).

Additional Info

Recognizes human USP13 at ~97kD. Species Crossreactivity: mouse

Read more on Supplier website

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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