USP18 (Ubiquitin Specific Protease 18, Ubiquitin-specific Protease 18, 43kD ISG15-specific Protease, ISG43, hUBP43, ISG15-specific-processing Protease, Ubl Carboxyl-terminal Hydrolase 18, Ubl Thioesterase 18)

UBP43, also known as ISG43 or USP18 is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first bona fide ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U4100-85

Size

100ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Tokarz,S., Berset,C., La Rue,J., Friedman,K., Nakayama,K., Nakayama,K., Zhang,D.E. and Lanker,S. (2004) The ISG15 isopeptidase UBP43\nis regulated by proteolysis via the SCFSkp2 ubiquitin ligase. J. Biol. Chem. 279 (45), 46424-46430.\nPuente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C. (2003) Human and mouse proteases: a comparative genomic approach. Nat.\nRev. Genet. 4 (7), 544-558.\nKang,D., Jiang,H., Wu,Q., Pestka,S. and Fisher,P.B. (2001) Cloning and characterization of human ubiquitin-processing protease-43 from\nterminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH. Gene 267 (2), 233-242.\nSchwer,H., Liu,L.Q., Zhou,L., Little,M.T., Pan,Z., Hetherington,C.J. and Zhang,D.E. (2000) Cloning and characterization of a novel human\nubiquitin-specific protease, a homologue of murine UBP43 (Usp18). Genomics 65 (1), 44-52.\nLi,X.L., Blackford,J.A., Judge,C.S., Liu,M., Xiao,W., Kalvakolanu,D.V. and Hassel,B.A. (2000) RNase-L-dependent destabilization of\ninterferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response. J. Biol. Chem. 275 (12), 8880-8888.

Additional Info

Species Crossreactivity: Crossreacts with Human.