Home  >  Products  >  USP18 (Ubiquitin Specific Protease 18, Ubiquitin-specific Protease 18, 43kD ISG15-specific Protease, ISG43, hUBP43, ISG15-specific-processing Protease, Ubl Carboxyl-terminal Hydrolase 18, Ubl Thioesterase 18)
USP18 (Ubiquitin Specific Protease 18, Ubiquitin-specific Protease 18, 43kD ISG15-specific Protease, ISG43, hUBP43, ISG15-specific-processing Protease, Ubl Carboxyl-terminal Hydrolase 18, Ubl Thioesterase 18)

USP18 (Ubiquitin Specific Protease 18, Ubiquitin-specific Protease 18, 43kD ISG15-specific Protease, ISG43, hUBP43, ISG15-specific-processing Protease, Ubl Carboxyl-terminal Hydrolase 18, Ubl Thioesterase 18)

Cat no: U4100-85

Supplier: United States Biological
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UBP43, also known as ISG43 or USP18 is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first bona fide ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U4100-85
Reactivities: Human
Hosts: Rabbit
Applications: ELISA
Size: 100ug
Form: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Tokarz,S., Berset,C., La Rue,J., Friedman,K., Nakayama,K., Nakayama,K., Zhang,D.E. and Lanker,S. (2004) The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase. J. Biol. Chem. 279 (45), 46424-46430. Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C. (2003) Human and mouse proteases: a comparative genomic approach. Nat. Rev. Genet. 4 (7), 544-558. Kang,D., Jiang,H., Wu,Q., Pestka,S. and Fisher,P.B. (2001) Cloning and characterization of human ubiquitin-processing protease-43 from terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH. Gene 267 (2), 233-242. Schwer,H., Liu,L.Q., Zhou,L., Little,M.T., Pan,Z., Hetherington,C.J. and Zhang,D.E. (2000) Cloning and characterization of a novel human ubiquitin-specific protease, a homologue of murine UBP43 (Usp18). Genomics 65 (1), 44-52. Li,X.L., Blackford,J.A., Judge,C.S., Liu,M., Xiao,W., Kalvakolanu,D.V. and Hassel,B.A. (2000) RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response. J. Biol. Chem. 275 (12), 8880-8888.
Additional info: Species Crossreactivity: Crossreacts with Human.

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