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Vacuolar Protein Sorting 28, Recombinant, Human (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)

Cat no: V0650-03

Vacuolar Protein Sorting 28, Recombinant, Human (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)

VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 , expressed in E. coli and purified by using conventional chromatography.\n\nSource:\nRecombinant human VPS28, aa1-221, expressed in E. coli\n\nAA Sequence:\nMFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V0650-03

Size

100ug

Form

Supplied as liquid in 20 mM Tris-HCl buffer, pH 7.5, 1 mM DTT, 10% Glycerol

Purity

~95% by SDS-PAGE Purified by immunoaffinity chromatography.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

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