VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VAMP-1, DKFZp686H12131, Synaptobrevin 1, Synaptobrevin-1, SYB1, SYB-1)

Supplier:

United States Biological

Cat no: V2024-01B

VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VAMP-1, DKFZp686H12131, Synaptobrevin 1, Synaptobrevin-1, SYB1, SYB-1)

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18kD member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa1-96) and a 22aa transmembrane domain (aa97-118). There is one coiled-coil region between aa33-93. Multiple splice variants are known that show two, three, four and 81aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. over aa1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2ug/ml\nImmunohistochemistry: 25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human VAMP-1 (aa1-96).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2024-01B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes endogenous human VAMP-1 and VAMP-2 at ~16kD. Does not react with recombinant VAMP family members 5, 7, 8. Species Crossreactivity: mouse.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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