VAMP 2, NT (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2, SYB2)

Synaptobrevin 2 (Syb2), also known as vesicle associated membrane protein-2 (VAMP2), is a 13kD membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. \n\nSynaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2,000-1:3,000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2024-02A

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Tajika, Y. et al. (2007) VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration. Cell Tissue Res. 328: 573-581. 2. Deak, F. et al. (2005) Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion. J. Neurosci. 26: 6668-6676. 3. Gerst, J.E. (1999) SNAREs and SNARE regulators in membrane fusion and exocytosis. Cell. Mol. Life Sci. 55: 707-734.

Additional Info

Recognizes human synaptobrevin 2. It also shows a low affinity to synaptobrevin 1. Species crossreactivity: rat.