VAMP 2 (Vesicle Associated Membrane Protein 2, Vesicle-associated Membrane Protein 2, VAMP2, VAMP-2, Synaptobrevin 2, Synaptobrevin-2, SYB 2, SYB2, SYB-2)

Synaptobrevins/VAMPs, syntaxins, and the 25kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is a member of the vesicle associated membrane protein (VAMP)/synaptobrevin family. VAMP2 is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. VAMP2 is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal associated protein, 25kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: AtT20 cells or any pooled pre-immune control.\n\nCellular Localization: Type IV membrane protein. Neuronal synaptic vesicles\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2024-02P

Size

50ug

Applications

ICC, IHC, WB

Hosts

Ch/Bird

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Schraw,T.D., et al. A role for Sec1/Munc18 proteins in platelet exocytosis. Biochem J. 2003 Aug 15;374(Pt 1):207-17.\n2. Martinez-Arca,S., et al. A dual mechanism controlling the localization and function of exocytic v-SNAREs. Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):9011-6. Epub 2003 Jul 9.\n3. Sun,W., et al. Hrs regulates early endosome fusion by inhibiting formation of an endosomal SNARE complex. J Cell Biol. 2003 Jul 7;162(1):125-37.\n4. Mollinedo,F., et al. Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils. J Immunol. 2003 Jan 15;170(2):1034-42.\n5. Takuma,T., et al. Trafficking of green fluorescent protein -tagged SNARE proteins in HSY cells. J Biochem (Tokyo). 2002 Nov;132(5):729-35.\n6. Sudhof, T.C., et al. The synaptic vesicle cycle: a cascade of protein-protein interactions. Nature. 376:64-5-53, 1995.

Additional Info

Species Crossreactivity: Crossreacts with Human, Mouse and Rat.